| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2014-01-29 |
| タイトル |
|
|
タイトル |
Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Titanium |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Stainless steel |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Dopamine |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Epidermal growth factor |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者 |
Kang, Jeonghwa
Sakuragi, Makoto
Shibata, Aya
Abe, Hiroshi
Kitajima, Takashi
Tada, Seiichi
Mizutani, Masayoshi
Ohmori, Hitoshi
Ayame, Hirohito
Son, Tae Il
Aigaki, Toshiro
Ito, Yoshihiro
|
| 著者別名 |
姜, 廷和
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Titanium and stainless steel were modified with dopamine for the immobilization of biomolecules, epidermal growth factor (EGF). First, the treatment of metal surfaces with a dopamine solution under different pH conditions was investigated. At higher pH, the dopamine solution turned brown and formed precipitates. Treatment of the metals with dopamine at pH 8.5 also resulted in the development of brown color at the surface of the metals. The hydrophobicity of the surfaces increased after treatment with dopamine, independently of pH. X-ray photoelectron spectroscopy revealed the formation of a significant amount of an organic layer on both surfaces at pH 8.5. According to ellipsometry measurements, the organic layer formed at pH 8.5 was about 1000 times as thick as that formed at pH 4.5. The amount of amino groups in the layer formed at pH 8.5 was also higher than that observed in the layer formed at pH 4.5. EGF molecules were immobilized onto the dopamine-treated surfaces via a coupling reaction using carbodiimide. A greater amount of EGF was immobilized on surfaces treated at pH 8.5 compared with pH 4.5. Significantly higher growth of rat fibroblast cells was observed on the two EGF-immobilized surfaces compared with non-immobilized surfaces in the presence of EGF. The present study demonstrated that metals can become bioactive via the surface immobilization of a growth factor and that the effect of the immobilized growth factor on metals was greater than that of soluble growth factor. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
首都大学東京, 2012-09-30, 博士(理学), 甲第320号 |
| 書誌情報 |
Materials Science and Engineering : C
巻 32,
号 8,
p. 2552-2561,
発行日 2012-12
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0928-4931 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
http://dx.doi.org/10.1016/j.msec.2012.07.039 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 国立国会図書館分類 |
|
|
主題Scheme |
NDLC |
|
主題 |
UT51 |
| 出版者 |
|
|
出版者 |
Elsevier B.V. |
| 関係URI |
|
|
|
識別子タイプ |
HDL |
|
|
関連識別子 |
http://hdl.handle.net/10748/6083 |
|
|
関連名称 |
http://hdl.handle.net/10748/6083 |
| 関係 |
|
|
|
関連名称 |
収録先の学位論文「Immobilization of Growth Factors on Inorganic Materials」 (http://hdl.handle.net/10748/6083) |
| 学位名 |
|
|
学位名 |
博士(理学) |
| 学位授与機関 |
|
|
|
学位授与機関名 |
首都大学東京 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2012-12 |
| 学位授与番号 |
|
|
学位授与番号 |
22604A320 |
T00175-001.pdf (2.1 MB)
