{"created":"2023-06-19T12:46:18.805719+00:00","id":6231,"links":{},"metadata":{"_buckets":{"deposit":"d5ad3432-50ee-47e6-a239-393cecf631a2"},"_deposit":{"created_by":3,"id":"6231","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6231"},"status":"published"},"_oai":{"id":"oai:tokyo-metro-u.repo.nii.ac.jp:00006231","sets":["656:687:688:1278:1446"]},"author_link":["20525","20526"],"item_2_alternative_title_19":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"カフェインは平滑筋ミオシンアクチン相互作用を抑制する"}]},"item_2_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"14","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_2_creator_2":{"attribute_name":"著者(ヨミ)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"オウ, シタン"}],"nameIdentifiers":[{"nameIdentifier":"20526","nameIdentifierScheme":"WEKO"}]}]},"item_2_date_granted_66":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-03-25"}]},"item_2_degree_grantor_64":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"首都大学東京"}]}]},"item_2_degree_name_63":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"修士（健康科学）"}]},"item_2_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Caffeine is known to have inhibitory effects on Ca^<2+>-induced contraction in smooth muscle, and possible mechanisms may contribute the inhibitory effects. Recently, it was found a caffeine effect on direct inhibition of myosin-actin interaction in smooth muscle. To clarify whether direct inhibition of myosin-actin interaction of caffeine to be dependent on the regulation of myosin light chain phosphorylation, in the present study, the author examined caffeine effects on skinned (cell membrane permeabilized) smooth muscle contraction. At 10 mM, caffeine significantly suppressed Ca^<2+> induced contraction, which was dependent on myosin light chain phosphorylation. On the other hand, caffeine did not affect 15.5 mM Mg^<2+> induced contraction, which was independent of myosin light chain phosphorylation. The present results suggest that caffeine inhibits actin-myosin interaction of smooth muscle through either inhibition of myosin light chain kinase activity or acceleration of myosin phosphatase activity.","subitem_description_type":"Abstract"},{"subitem_description":"カフェインには平滑筋にCaイオンの抑制があることが知られている。様々な機構がこの抑制に関与している。最近、カフェインは平滑筋のミオシンアクチン相互作用を直接的に抑制することが明らかになった。本研究では、細胞膜を破壊したスキンド盲腸紐標本を用いて、カフェインの平滑筋アクチン・ミオシン相互作用の抑制が、ミオシン軽鎖リン酸化の抑制によるものなのかを検討した。10mMのカェインはミオシン軽鎖リン酸化に依存するCaイオン活性化収縮張力を抑制した。一方、カフェインはミオシン軽鎖リン酸化に依存しない15.5mM Mgイオンによる収縮張力を抑制しない。以上から、カフェインはミオシン軽鎖リン酸化酵素の活性阻害、もしくはミオシンボスファターゼの活性を促進することでミオシン軽鎖リン酸化レベルを低下させることで、平滑筋ミオシンアクチン相互作用を抑制し、平滑筋収縮を抑制することが示唆された。","subitem_description_type":"Abstract"}]},"item_2_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"首都大学東京, 2017-03-25, 修士（健康科学）","subitem_description_type":"Other"}]},"item_2_version_type_16":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"王, 志丹"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-15"}],"displaytype":"detail","filename":"T01112-001.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"T01112-001.pdf","url":"https://tokyo-metro-u.repo.nii.ac.jp/record/6231/files/T01112-001.pdf"},"version_id":"931f56a1-9071-4ce0-a6e6-dbcd7c339d5f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"caffeine","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca^<2+> -induced contraction","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin-actin interaction","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin light chain phosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"カフェイン","subitem_subject_scheme":"Other"},{"subitem_subject":"Ca^<2+>活性化収縮","subitem_subject_scheme":"Other"},{"subitem_subject":"ミオシン-アクチン相互作用","subitem_subject_scheme":"Other"},{"subitem_subject":"ミオシン軽鎖リン酸化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Caffeine inhibits smooth muscle actin-myosin interaction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Caffeine inhibits smooth muscle actin-myosin interaction"}]},"item_type_id":"2","owner":"3","path":["1446"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-29"},"publish_date":"2017-09-29","publish_status":"0","recid":"6231","relation_version_is_last":true,"title":["Caffeine inhibits smooth muscle actin-myosin interaction"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T16:44:52.710929+00:00"}